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SIDT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SIDT1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112282-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIDT1 (NM_017699), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SIDT1 | SID-1 | SID1
Origin Species
Human
NCBI GenBank
NM_017699
SwissProt
Q9NXL6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRGCLRLALL CALPWLLLAA SPGHPAKSPR QPPAPRRDPF DAARGADFDH VYSGVVNLST
61ENIYSFNYTS QPDQVTAVRV YVNSSSENLN YPVLVVVRQQ KEVLSWQVPL LFQGLYQRSY
121NYQEVSRTLC PSEATNETGP LQQLIFVDVA SMAPLGAQYK LLVTKLKHFQ LRTNVAFHFT
181ASPSQPQYFL YKFPKDVDSV IIKVVSEMAY PCSVVSVQNI MCPVYDLDHN VEFNGVYQSM
241TKKAAITLQK KDFPGEQFFV VFVIKPEDYA CGGSFFIQEK ENQTWNLQRK KNLEVTIVPS
301IKESVYVKSS LFSVFIFLSF YLGCLLVGFV HYLRFQRKSI DGSFGSNDGS GNMVASHPIA
361ASTPEGSNYG TIDESSSSPG RQMSSSDGGP PGQSDTDSSV EESDFDTMPD IESDKNIIRT
421KMFLYLSDLS RKDRRIVSKK YKIYFWNIIT IAVFYALPVI QLVITYQTVV NVTGNQDICY
481YNFLCAHPLG VLSAFNNILS NLGHVLLGFL FLLIVLRRDI LHRRALEAKD IFAVEYGIPK
541HFGLFYAMGI ALMMEGVLSA CYHVCPNYSN FQFDTSFMYM IAGLCMLKLY QTRHPDINAS
601AYSAYASFAV VIMVTVLGVV FGKNDVWFWV IFSAIHVLAS LALSTQIYYM GRFKIDLGIF
661RRAAMVFYTD CIQQCSRPLY MDRMVLLVVG NLVNWSFALF GLIYRPRDFA SYMLGIFICN
721LLLYLAFYII MKLRSSEKVL PVPLFCIVAT AVMWAAALYF FFQNLSSWEG TPAESREKNR
781ECILLDFFDD HDIWHFLSAT ALFFSFLVLL TLDDDLDVVR RDQIPVF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

827 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.