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SIAE Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SIAE Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105642-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIAE (NM_170601), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVAPGLVLGL VLPLILWADR SAGIGFRFAS YINNDMVLQK EPAGAVIWGF GTPGATVTVT
61LRQGQETIMK KVTSVKAHSD TWMVVLDPMK PGGPFEVMAQ QTLEKINFTL RVHDVLFGDV
121WLCSGQSNMQ MTVLQIFNAT RELSNTAAYQ SVRILSVSPI QAEQELEDLV AVDLQWSKPT
181SENLGHGYFK YMSAVCWLFG RHLYDTLQYP IGLIASSWGG TPIEAWSSGR SLKACGVPKQ
241GSIPYDSVTG PSKHSVLWNA MIHPLCNMTL KGVVWYQGES NINYNTDLYN CTFPALIEDW
301RETFHRGSQG QTERFFPFGL VQLSSDLSKK SSDDGFPQIR WHQTADFGYV PNPKMPNTFM
361AVAMDLCDRD SPFGSIHPRD KQTVAYRLHL GARALAYGEK NLTFEGPLPE KIELLAHKGL
421LNLTYYQQIQ VQKKDNKIFE ISCCSDHRCK WLPASMNTVS TQSLTLAIDS CHGTVVALRY
481AWTTWPCEYK QCPLYHPSSA LPAPPFIAFI TDQGPGHQSN VAK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

523 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.