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SHROOM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SHROOM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SHROOM1 antibodies →
$725.00each
Catalog Number: LS-G107501-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHROOM1 (NM_133456), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Apical protein 2 | APXL2 | KIAA1960 | SHROOM1 | Protein Shroom1 | Shroom family member 1
Origin Species
Human
SwissProt
Q2M3G4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEALGPGGDR ASPASSTSSL DLWHLSMRAD SAYSSFSAAS GGPEPRTQSP GTDLLPYLDW
61DYVRVVWGGP GPAPPDAALC TSPRPRPAVA ARSGPQPTEV PGTPGPLNRQ ATPLLYALAA
121EAEAAAQAAE PPSPPASRAA YRQRLQGAQR RVLRETSFQR KELRMSLPAR LRPTVPARPP
181ATHPRSASLS HPGGEGEPAR SRAPAPGTAG RGPLANQQRK WCFSEPGKLD RVGRGGGPAR
241ECLGEACSSS GLPGPEPLEF QHPALAKFED HEVGWLPETQ PQGSMNLDSG SLKLGDAFRP
301ASRSRSASGE VLGSWGGSGG TIPIVQAVPQ GAETPRPLFQ TKLSRFLPQK EAAVMYPAEL
361PQSSPADSEQ RVSETCIVPA WLPSLPDEVF LEEAPLVRMR SPPDPHASQG PPASVHASDQ
421PYGTGLGQRT GQVTVPTEYP LHECPGTAGA DDCWQGVNGS VGISRPTSHT PTGTANDNIP
481TIDPTGLTTN PPTAAESDLL KPVPADALGL SGNDTPGPSH NTALARGTGQ PGSRPTWPSQ
541CLEELVQELA RLDPSLCDPL ASQPSPEPPL GLLDGLIPLA EVRAAMRPAC GEAGEEAAST
601FEPGSYQFSF TQLLPAPREE TRLENPATHP VLDQPCGQGL PAPNNSIQGK KVELAARLQK
661MLQDLHTEQE RLQGEAQAWA RRQAALEAAV RQACAPQELE RFSRFMADLE RVLGLLLLLG
721SRLARVRRAL ARAASDSDPD EQRLRLLQRQ EEDAKELKEH VARRERAVRE VLVRALPVEE
781LRVYCALLAG KAAVLAQQRN LDERIRLLQD QLDAIRDDLG HHAPSPSPAR PPGTCPPVQP
841PFPLLLT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

847 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.