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SHQ1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SHQ1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111959-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHQ1 (NM_018130), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SHQ1 homolog (S. cerevisiae) | Protein SHQ1 homolog | SHQ1 | Shq1p
Origin Species
Human
NCBI GenBank
NM_018130
SwissProt
Q6PI26

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLTPAFDLSQ DPDFLTIAIR VPYARVSEFD VYFEGSDFKF YAKPYFLRLT LPGRIVENGS
61EQGSYDADKG IFTIRLPKET PGQHFEGLNM LTALLAPRKS RTAKPLVEEI GASEIPEEVV
121DDEEFDWEIE QTPCEEVSES ALNPQCHYGF GNLRSGVLQR LQDELSDVID IKDPDFTPAA
181ERRQKRLAAE LAKFDPDHYL ADFFEDEAIE QILKYNPWWT DKYSKMMAFL EKSQEQENHA
241TLVSFSEEEK YQLRKFVNKS YLLDKRACRQ VCYSLIDILL AYCYETRVTE GEKNVESAWN
301IRKLSPTLCW FETWTNVHDI MVSFGRRVLC YPLYRHFKLV MKAYRDTIKI LQLGKSAVLK
361CLLDIHKIFQ ENDPAYILND LYISDYCVWI QKVKSKKLAA LAEALKEVSL TKAQLGLELE
421ELEAAALLVQ EEETALKAAH SVSGQQTLCS SSEASDSEDS DSSVSSGNED SGSDSEQDEL
481KDSPSETVSS LQGPFLEESS AFLIVDGGVR RNTAIQESDA SQGKPLASSW PLGVSGPLIE
541ELGEQLKTTV QVSEPKGTTA VNRSNIQERD GCQTPNN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

577 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.