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SHKBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SHKBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107365-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHKBP1 (NM_138392), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Sb1 | SHKBP1 | SH3KBP1 binding protein 1 | SH3KBP1-binding protein 1 | PP203
Origin Species
Human
NCBI GenBank
NM_138392
SwissProt
Q8TBC3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAATAAEG VPSRGPPGEV IHLNVGGKRF STSRQTLTWI PDSFFSSLLS GRISTLKDET
61GAIFIDRDPT VFAPILNFLR TKELDPRGVH GSSLLHEAQF YGLTPLVRRL QLREELDRSS
121CGNVLFNGYL PPPVFPVKRR NRHSLVGPQQ LGGRPAPVRR SNTMPPNLGN AGLLGRMLDE
181KTPPSPSGQP EEPGMVRLVC GHHNWIAVAY TQFLVCYRLK EASGWQLVFS SPRLDWPIER
241LALTARVHGG ALGEHDKMVA AATGSEILLW ALQAEGGGSE IGVFHLGVPV EALFFVGNQL
301IATSHTGRIG VWNAVTKHWQ VQEVQPITSY DAAGSFLLLG CNNGSIYYVD VQKFPLRMKD
361NDLLVSELYR DPAEDGVTAL SVYLTPKTSD SGNWIEIAYG TSSGGVRVIV QHPETVGSGP
421QLFQTFTVHR SPVTKIMLSE KHLISVCADN NHVRTWSVTR FRGMISTQPG STPLASFKIL
481ALESADGHGG CSAGNDIGPY GERDDQLVFI QKVVPSASQL FVRLSSTGQR VCSVRSVDGS
541PTTAFTVLEC EGSRRLGSRP RRYLLTGQAN GSLAMWDLTT AMDGLGQAPA GGLTEQELME
601QLEHCELAPP APSAPSWGCL PSPSPRISLT SLHSASSNTS LSGHRGSPSP PQAEARRRGG
661GSFVERCQEL VRSGPDLRRP PTPAPWPSSG LGTPLTPPKM KLNETSF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

707 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.