Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SHFM3 / FBXW4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110506-100
Catalog Number: LS-G110506
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHFM3(FBXW4) (NM_022039), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DAC | Dactylin | FBW4 | FBWD4 | FBXW4 | F-box/WD repeat protein 4 | SHFM3 | SHSF3
- Origin Species
- Human
- NCBI GenBank
- NM_022039
- SwissProt
- P57775
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAAAGEEEE EEEAARESAA RPAAGPALWR LPEELLLLIC SYLDMRALGR LAQVCRWLRR61FTSCDLLWRR IARASLNSGF TRLGTDLMTS VPVKERVKVS QNWRLGRCRE GILLKWRCSQ121MPWMQLEDDS LYISQANFIL AYQFRPDGAS LNRRPLGVFA GHDEDVCHFV LANSHIVSAG181GDGKIGIHKI HSTFTVKYSA HEQEVNCVDC KGGIIVSGSR DRTAKVWPLA SGRLGQCLHT241IQTEDRVWSI AISPLLSSFV TGTACCGHFS PLRIWDLNSG QLMTHLGSDF PPGAGVLDVM301YESPFTLLSC GYDTYVRYWD LRTSVRKCVM EWEEPHDSTL YCLQTDGNHL LATGSSYYGV361VRLWDRRQRA CLHAFPLTST PLSSPVYCLR LTTKHLYAAL SYNLHVLDFQ NPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
412 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.