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SHF Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SHF Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107337-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHF (NM_138356), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SHF
Origin Species
Human
NCBI GenBank
NM_138356
SwissProt
Q7M4L6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MQQEGGPVRS APCRTGTLEG SRQGSPGHRK RASPKGSLSS AQPHSWMLTP SPLNSHCAHR
61EPISSSPQPV ANGPKQKKKS NWRSTTRLRI IRLRDRLEPR PLAILEDYAD PFDVQETGEG
121SAGASGAPEK VPENDGYMEP YEAQKMMAEI RGSKETATQP LPLYDTPYEP EEDGATPEGE
181GAPWPRESRL PEDDERPPEE YDQPWEWKKE RISKAFAVDI KVIKDLPWPP PVGQLDSSPS
241LPDGDRDISG PASPLPEPSL EDSSAQFEGP EKSCLSPGRE EKGRLPPRLS AGNPKSAKPL
301SMEPSSPLGE WTDPALPLEN QVWYHGAISR TDAENLLRLC KEASYLVRNS ETSKNDFSLS
361LKSSQGFMHM KLSRTKEHKY VLGQNSPPFS SVPEIVHHYA SRKLPIKGAE HMSLLYPVAI
421RTL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

423 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.