LSBio
SHE Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SHE Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SHE antibodies →
$725.00each
Catalog Number: LS-G121690-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHE (NM_001010846), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001010846

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MQWSPTPGAS ACLGWASSLA CSTAPTLLGR AGRGPLMAAK WFKEFPLNLK TVSERAKPGG
61GGGKLRKNSE AGGAGPGPGK GRKNSAAELG SGRAGVGPKD SRLSRDSLQG LIQAAAGKGR
121KNSRATEEEP HRGATKSSGC STYINRLIKV DTQEKNGKSN YPSSSSSSSS SSSSASSSPS
181SLGPELDKGK IIKQQETVII LEDYADPYDA KRTKGQRDAE RVGENDGYME PYDAQQMITE
241IRRRGSKDPL VKALQLLDSP CEPADGGLKS ETLAKRRSSK DLLGKPPQLY DTPYEPAEGG
301PRAEGKARPP DSRLPENDER PAAEYEQPWE WKKEQIVRAL SVQFEGAERP SFREETVRQH
361HRQKSWTQKI LKPALSDHSE GEKVDPGLPL EKQPWYHGAI SRAEAESRLQ PCKEAGYLVR
421NSESGNSRYS IALKTSQGCV HIIVAQTKDN KYTLNQTSAV FDSIPEVVHY YSNEKLPFKG
481AEHMTLLYPV HSKLH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

495 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.