Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SHCBP1L / C1orf14 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109343-100
Catalog Number: LS-G109343
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHCBP1L (NM_030933), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C1orf14 | GE36 | SHCBP1L
- Origin Species
- Human
- NCBI GenBank
- NM_030933
- SwissProt
- Q9BZQ2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASGSKASVP ADSFRTISPD RRGEKSASAV SGDTAAATTL KGTAIPVRSV VASPRPVKGK61AGRETARLRL QRLPAAQAED TGEAAAAAAE EPLLPVPEDE EEAQPLPPVC VSRMRGMWRD121EKVSLYCDEV LQDCKAEDAD EVMGKYLSEK LKLKDKWLGV WKTNPSVFFV KYEEASIPFV181GILVEVTCEP YQDSSSRFKV TVSVAEPFSS NIANIPRDLV DEILEELEHS VPLLEVYPVE241GQDTDIHVIA LALEVVRFFY DFLWRDWDDE ESCENYTALI EERINLWCDI QDGTIPGPIA301QRFKKTLEKY KNKRVELIEY QSNIKEDPSA AEAVECWKKY YEIVMLCGLL KMWEDLRLRV361HGPFFPRILR RRKGKREFGK TITHIVAKMM TTEMIKDLSS DTLLQQHGDL DLALDNCYSG421DTVIIFPGEY QAANLALLTD DIIIKGVGKR EEIMITSEPS RDSFVVSKAD NVKLMHLSLI481QQGTVDGIVV VESGHMTLEN CILKCEGTGV CVLTGAALTI TDSEITGAQG AGVELYPGSI541AILERNEIHH CNNLRTSNSS KSTLGGVNMK VLPAPKLKMT NNHIYSNKGY GVSILQPMEQ601FFIVAEEALN KRASSGDKKD DKMLFKVMQN LNLEMNNNKI EANVKGDIRI VTSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
653 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.