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SHCBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SHCBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SHCBP1 antibodies →
$504.00each
Catalog Number: LS-G109786-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SHCBP1 (NM_024745), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PAL | SHCBP1
Origin Species
Human
NCBI GenBank
NM_024745
SwissProt
Q8NEM2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADGSLTGGG LEAAAMAPER TGWAVEQELA SLEKGLFQDE DSCSDCSYRD KPGSSLQSFM
61PEGKTFFPEI FQTNQLLFYE RFRAYQDYIL ADCKASEVQE FTAEFLEKVL EPSGWRAVWH
121TNVFKVLVEI TDVDFAALKA VVRLAEPYLC DSQVSTFTME CMKELLDLKE HRLPLQELWV
181VFDDSGVFDQ TALAIEHVRF FYQNIWRSWD EEEEDEYDYF VRCVEPRLRL HYDILEDRVP
241SGLIVDYHNL LSQCEESYRK FLNLRSSLSN CNSDSEQENI SMVEGLKLYS EMEQLKQKLK
301LIENPLLRYV FGYQKNSNIQ AKGVRSSGQK ITHVVSSTMM AGLLRSLLTD RLCQEPGEEE
361REIQFHSDPL SAINACFEGD TVIVCPGHYV VHGTFSIADS IELEGYGLPD DIVIEKRGKG
421DTFVDCTGAD IKISGIKFVQ HDAVEGILIV HRGKTTLENC VLQCETTGVT VRTSAEFLMK
481NSDLYGAKGA GIEIYPGSQC TLSDNGIHHC KEGILIKDFL DEHYDIPKIS MVNNIIHNNE
541GYGVVLVKPT IFSDLQESAE DGTEENKALK IQTSGEPDVA ERVDLEELIE CATGKMELCA
601RTDPSEQVEG NCEIVNELIA ASTQKGQIKK KRLSELGITQ ADDNLMSQEM FVGIVGNQFK
661WNGKGSFGTF LF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

672 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.