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SH3TC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SH3TC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SH3TC1 antibodies →
$725.00each
Catalog Number: LS-G111507-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SH3TC1 (NM_018986), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SH3TC1
Origin Species
Human
NCBI GenBank
NM_018986
SwissProt
Q8TE82

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
146.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENLPAVTTE EPTPMGRGPV GPSGGGSTRD QVRTVVMRPS VSWEKAGPEE AKAPVRGDEA
61PPARVAGPAA GTPPCQMGVY PTDLTLQLLA VRRKSRLRDP GLQQTLRGQL RLLENDSREM
121ARVLGELSAR LLSIHSDQDR IVVTFKTFEE IWKFSTYHAL GFTHHCLANL LMDQAFWLLL
181PSEEEETAIQ VHVDENALRL THESLLIQEG PFFVLCPDHH VRVMTGPRDA GNGPQALRQA
241SGAPQGEAAP ETDSSPPSPS VSSEEVAVAA APEPLIPFHQ WALRIPQDPI DDAMGGPVMP
301GNPLMAVGLA SALADFQGSG PEEMTFRGGD LIEILGAQVP SLPWCVGRHA ASGRVGFVRS
361SLISMQGPVS ELESAIFLNE EEKSFFSEGC FSEEDARQLL RRMSGTDVCS VYSLDSVEEA
421ETEQPQEKEI PPPCLSPEPQ ETLQKVKNVL EQCKTCPGCP QEPASWGLCA ASSDVSLQDP
481EEPSFCLEAE DDWEDPEALS SLLLFLNAPG YKASFRGLYD VALPWLSSVF RSFSDEEELT
541GRLAQARGAA KKAGLLMALA RLCFLLGRLC SRRLKLSQAR VYFEEALGAL EGSFGDLFLV
601VAVYANLASI YRKQKNREKC AQVVPKAMAL LLGTPDHICS TEAEGELLQL ALRRAVGGQS
661LQAEARACFL LARHHVHLKQ PEEALPFLER LLLLHRDSGA PEAAWLSDCY LLLADIYSRK
721CLPHLVLSCV KVASLRTRGS LAGSLRSVNL VLQNAPQPHS LPAQTSHYLR QALASLTPGT
781GQALRGPLYT SLAQLYSHHG CHGPAITFMT QAVEASAIAG VRAIVDHLVA LAWLHVLHGQ
841SPVALDILQS VRDAVVASED QEGVIANMVA VALKRTGRTR QAAESYYRAL RVARDLGQQR
901NQAVGLANFG ALCLHAGASR LAQHYLLEAV RLFSRLPLGE CGRDFTHVLL QLGHLCTRQG
961PAQQGKGYYE WALLVAVEMG HVESQLRAVQ RLCHFYSAVM PSEAQCVIYH ELQLSLACKV
1021ADKVLEGQLL ETISQLYLSL GTERAYKSAL DYTKRSLGIF IDLQKKEKEA HAWLQAGKIY
1081YILRQSELVD LYIQVAQNVA LYTGDPNLGL ELFEAAGDIF FDGAWEREKA VSFYRDRALP
1141LAVTTGNRKA ELRLCNKLVA LLATLEEPQE GLEFAHMALA LSITLGDRLN ERVAYHRLAA
1201LQHRLGHGEL AEHFYLKALS LCNSPLEFDE ETLYYVKVYL VLGDIIFYDL KDPFDAAGYY
1261QLALAAAVDL GNKKAQLKIY TRLATIYHNF LLDREKSLFF YQKARTFATE LNVRRVNLPP
1321LPLCGWAPWL APSHPR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1336 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.