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SH3RF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SH3RF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119059-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SH3RF3 (NM_001099289), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Plenty of SH3s 2 | Plenty of SH3s-2 | POSH2 | SH3 multiple domains 4 | SH3MD4 | SH3 multiple domains protein 4 | SH3RF3
Origin Species
Human
SwissProt
Q8TEJ3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLGASWLCA SKAAAAAAQS EGDEDRPGER RRRRAAATAA GAGEDMDESS LLDLLECSVC
61LERLDTTAKV LPCQHTFCRR CLESIVCSRH ELRCPECRIL VGCGVDELPA NILLVRLLDG
121IRQRPRAGTS PGGSPPARPI PGQSAAPTLA GGGGGAAGST PGSPVFLSAA AGSTAGSLRE
181LATSRTAPAA KNPCLLPYGK ALYSYEGKEP GDLKFNKGDI IVLRRKVDEQ WYHGELHGTQ
241GFLPASYIQC IQPLPHAPPQ GKALYDFEMK DKDQDKDCLT FTKDEILTVL RRVDENWAEG
301MLGDKIGIFP LLYVELNDSA KQLIEMDKPC PAAASSCNAS LPSDSGAVAS VAPSPTLSSS
361GAVSAFQRRV DGKKNTKKRH SFTALSVTHR SSQAASHRHS MEISAPVLIS SSDPRAAARI
421GDLAHLSCAA PTQDVSSSAG STPTAVPRAA SVSGEQGTPP KVQLPLNVYL ALYAYKPQKS
481DELELHKGEM YRVLEKCQDG WFKGASLRTG VSGVFPGNYV TPVSRVPAGG AGPPRNNVVG
541GSPLAKGITT TMHPGSGSLS SLATATRPAL PITTPQAHAQ HPTASPPTGS CLRHSAQPTA
601SQARSTISTA AHSAAQAQDR PTATVSPLRT QNSPSRLPAT SLRPHSVVSP QHSHQPPVQM
661CPRPAIPLTS AASAITPPNV SAANLNGEAG GGPIGVLSTS SPTNTGCKLD EKKSEKKEKK
721SGLLKLLAGA STKKKSRSPP SVSPTHDPQV AVDALLQGAV GPEVSSLSIH GRAGSCPIES
781EMQGAMGMEP LHRKAGSLDL NFTSPSRQAP LSMAAIRPEP KLLPRERYRV VVSYPPQSEA
841EIELKEGDIV FVHKKREDGW YKGTLQRNGR TGLFPGSFVE SF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

882 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.