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SH3RF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SH3RF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106187-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF158(SH3RF2) (NM_152550), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HEPP1 | PPP1R39 | POSH-eliminating RING protein | RING finger protein 158 | RNF158 | SH3RF2 | POSHER
Origin Species
Human
NCBI GenBank
W86728

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDDLTLLDLL ECPVCFEKLD VTAKVLPCQH TFCKPCLQRV FKAHKELRCP ECRTPVFSNI
61EALPANLLLV RLLDGVRSGQ SSGRGGSFRR PGTMTLQDGR KSRTNPRRLQ ASPFRLVPNV
121RIHMDGVPRA KALCNYRGQN PGDLRFNKGD IILLRRQLDE NWYQGEINGI SGNFPASSVE
181VIKQLPQPPP LCRALYNFDL RGKDKSENQD CLTFLKDDII TVISRVDENW AEGKLGDKVG
241IFPILFVEPN LTARHLLEKN KGRQSSCTKN LSLVSSSSRG NTSTLRRGPG SRRKVPGQFS
301ITTALNTLNR MVHSPSGRHM VEISTPVLIS SSNPSVITQP MEKADVPSSC VGQVSTYHPA
361PVSPGHSTAV VSLPGSQQHL SANMFVALHS YSAHGPDELD LQKGEGVRVL GKCQDGWLRG
421VSLVTGRVGI FPNNYVIPIF RKTSSFPDSR SPGLYTTWTL STSSVSSQGS ISEGDPRQSR
481PFKSVFVPTA IVNPVRSTAG PGTLGQGSLR KGRSSMRKNG SLQRPLQSGI PTLVVGSLRR
541SPTMVLRPQQ FQFYQPQGIP SSPSAVVVEM GSKPALTGEP ALTCISRGSE ARIHSAASSL
601IMEDKEIPIK SEPLPKPPAS APPSILVKPE NSRNGIEKQV KTVRFQNYSP PPTKHYTSHP
661TSGKPEQPAT LKASQPEAAS LGPEMTVLFA HRSGCHSGQQ TDLRRKSALA KATTLVSTAS
721GTQTVFPSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

729 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.