Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SH3P7 / HIP-55 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121574-100
Catalog Number: LS-G121574
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HIP55(DBNL) (NM_001014436), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DBNL | Drebrin-like | HIP-55 | SH3P7 | Cervical SH3P7 | CMAP | Drebrin-F | Drebrin-like protein
- Origin Species
- Human
- NCBI GenBank
- NM_014063
- SwissProt
- Q9UJU6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAANLSRNGP ALQEAYVRVV TEKSPTDWAL FTYEGNSNDI RVAGTGEGGL EEMVEELNSG61KVMYAFCRVK DPNSGLPKFV LINWTGEGVN DVRKGACASH VSTMASFLKG AHVTINARAE121EDVEPECIME KVAKASGANY SFHKESGRFQ DVGPQAPVGS VYQKTNAVSE IKRVGKDSFW181AKAEKEEENR RLEEKRRAEE AQRQLEQERR ERELREAARR EQRYQEQGGE ASPQSRTWEQ241QQEVVSRNRN EQESAVHPRE IFKQKERAMS TTSISSPQPG KLRSPFLQKQ LTQPETHFGR301EPAAAISRPR ADLPAEEPAP STPPCLVQAE EEAVYEEPPE QETFYEQPPL VQQQGAGSEH361IDHHIQGQGL SGQGLCARAL YDYQAADDTE ISFDPENLIT GIEVIDEGWW RGYGPDGHFG421MFPANYVELI ETRTRPLEQKLISEEDLAANDILDYKDDDDKV
431 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.