Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SH3KBP1 / CIN85 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121071-100
Catalog Number: LS-G121071
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SH3KBP1 (NM_001024666), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD2BP3 | CIN85 | GIG10 | HSB-1 | HSB1 | C-Cbl-interacting protein | CD2-binding protein 3 | MIG18 | Migration-inducing gene 18 | SH3KBP1
- Origin Species
- Human
- NCBI GenBank
- NM_031892
- SwissProt
- Q96B97
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEVSAAKAPS AADLSEIEIK KEMKKDPLTN KAPEKPLHEV PSGNSLLSSE TILRTNKRGE61RRRRRCQVAF SYLPQNDDEL ELKVGDIIEV VGEVEEGWWE GVLNGKTGMF PSNFIKELSG121ESDELGISQD EQLSKSSLRE TTGSESDGGD SSSTKSEGAN GTVATAAIQP KKVKGVGFGD181IFKDKPIKLR PRSIEVENDF LPVEKTIGKK LPATTATPDS SKTEMDSRTK SKDYCKVIFP241YEAQNDDELT IKEGDIVTLI NKDCIDVGWW EGELNGRRGV FPDNFVKLLP PDFEKEGNRP301KKPPPPSAPV IKQGAGTTER KHEIKKIPPE RPEMLPNRTE EKERPEREPK LDLQKPSVPA361IPPKKPRPPK TNSLSRPGAL PPRRPERPVG PLTHTRGDSP KIDLAGSSLS GILDKDLSDR421SNDIDLEGFD SVVSSTEKLS HPTTSRPKAT GRRPPSQSLT SSSLSSPDIF DSPSPEEDKE481EHISLAHRGV DASKKTSKTV TISQVSDNKA SLPPKPGTMA AGGGGPAPLS SAAPSPLSSS541LGTAGHRANS PSLFGTEGKP KMEPAASSQA AVEELRTQVR ELRSIIETMK DQQKREIKQL601LSELDEEKKI RLRLQMEVND IKKALQSKTR RLEQKLISEEDLAANDILDYKDDDDKV
631 aa + 26 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.