Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SH3GLB2 / Endophilin-B2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101651-100
Catalog Number: LS-G101651
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SH3GLB2 (NM_020145), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Endophilin-B2 | PP6569 | SH3GLB2 | SH3-containing protein SH3GLB2 | RRIG1 | KIAA1848 | PP9455
- Origin Species
- Human
- NCBI GenBank
- NM_020145
- SwissProt
- Q9NR46
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDFNMKKLAS DAGIFFTRAV QFTEEKFGQA EKTELDAHFE NLLARADSTK NWTEKILRQT61EVLLQPNPSA RVEEFLYEKL DRKVPSRVTN GELLAQYMAD AASELGPTTP YGKTLIKVAE121AEKQLGAAER DFIHTASISF LTPLRNFLEG DWKTISKERR LLQNRRLDLD ACKARLKKAK181AAEAKATTVP DFQETRPRNY ILSASASALW NDEVDKAEQE LRVAQTEFDR QAEVTRLLLE241GISSTHVNHL RCLHEFVKSQ TTYYAQCYRH MLDLQKQLGR FPGTFVGTTE PASPPLSSTS301PTTAAATMPV VPSVASLAPP GEASLCLEEV APPASGTRKA RVLYDYEAAD SSELALLADE361LITVYSLPGM DPDWLIGERG NKKGKVPVTY LELLSTRTRP LEQKLISEED LAANDILDYK421DDDDKV
426 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.