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SH2D5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SH2D5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G118847-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SH2D5 (NM_001103161), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SH2D5 | SH2 domain containing 5
Origin Species
Human
NCBI GenBank
NM_001103160
SwissProt
Q6ZV89

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MQKAGAGGRR ASDCGLAPHR PRCITKFAQY VGSFPVDDLD TQESVWLVQQ QLWALKDCPR
61RRAVILKFSL QGLKIYSGEG EVLLMAHALR RILYSTWCPA DCQFAFMARN PRSPASKLFC
121HLFVGSQPGE VQILHLLLCR SFQLAYLLQH PEERAQPEPC PGPTGEVPLK PLSSSGGLVR
181EPFGRDQLSQ NVHALVSFRR LPAEGLVGSG KELPESEGRA RHARLGNPYC SPTLVRKKAI
241RSKVIRSGAY RGCTYETQLQ LSAREAFPAA WEAWPRGPGG HSCLVESEGS LTENIWAFAG
301ISRPCALALL RRDVLGAFLL WPELGASGQW CLSVRTQCGV VPHQVFRNHL GRYCLEHLPA
361EFPSLEALVE NHAVTERSLF CPLDMGRLNP TYEEQDCGPP GRPPRTLRPL SHAKSEAELQ
421GLG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

423 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.