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SGSM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SGSM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SGSM2 antibodies →
$725.00each
Catalog Number: LS-G119235-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SGSM2 (NM_001098509), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0397 | RUTBC1 | SGSM2
Origin Species
Human
NCBI GenBank
AB007857
SwissProt
O43147

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSAEDAVKE KLLWNVKKEV KQIMEEAVTR KFVHEDSSHI IALCGAVEAC LLHQLRRRAA
61GFLRSDKMAA LFTKVGKTCP VAGEICHKVQ ELQQQAEGRK PSGVSQEALR RQGSASGKAP
121ALSPQALKHV WVRTALIEKV LDKVVQYLAE NCSKYYEKEA LLADPVFGPI LASLLVGPCA
181LEYTKLKTAD HYWTDPSADE LVQRHRIRGP PTRQDSPAKR PALGIRKRHS SGSASEDRLA
241ACARECVESL HQNSRTRLLY GKNHVLVQPK EDMEAVPGYL SLHQSAESLT LKWTPNQLMN
301GTLGDSELEK SVYWDYALVV PFSQVVCIHC HQQKSGGTLV LVSQDGIQRP PLHFPQGGHL
361LSFLSCLENG LLPRGQLEPP LWTQQGKGKV FPKLRKRSSI RSVDMEEMGT GRATDYVFRI
421IYPGHRHEHN AGDMIEMQGF GPSLPAWHLE PLCSQGSSCL SCSSSSSPHA TPSHCSCIPD
481RLPLRLLCES MKRQIVSRAF YGWLAHCRHL STVRTHLSAL VHHSVIPPDR PPGASAGLTK
541DVWSKYQKDK KNYKELELLR QVYYGGIEHE IRKDVWPFLL GHYKFGMSKK EMEQVDAVVA
601ARYQQVLAEW KACEVVVRQR EREAHPATRT KFSSGSSIDS HVQRLIHRDS TISNDVFISV
661DDLEPPEPQD PEDSRPKPEQ EAGPGTPGTA VVEQQHSVEF DSPDSGLPSS RNYSVASGIQ
721SSLDEGQSVG FEEEDGGGEE GSSGPGPAAH TLREPQDPSQ EKPQAGELEA GEELAAVCAA
781AYTIELLDTV ALNLHRIDKD VQRCDRNYWY FTPPNLERLR DVMCSYVWEH LDVGYVQGMC
841DLLAPLLVTL DNDQLAYSCF SHLMKRMSQN FPNGGAMDTH FANMRSLIQI LDSELFELMH
901QNGDYTHFYF CYRWFLLDFK RELLYEDVFA VWEVIWAARH ISSEHFVLFI ALALVEAYRE
961IIRDNNMDFT DIIKFFNERA EHHDAQEILR IARDLVHKVQ MLIENK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1006 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.