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SFRP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SFRP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117608-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SFRP5 (NM_003015), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FRP-1b | Frizzled-related protein 5 | SFRP-5 | SARP-3 | Frizzled-related protein 1b | FRP1B | SARP3 | SFRP5
Origin Species
Human
NCBI GenBank
NM_003015
SwissProt
Q5T4F7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
35.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MRAAAAAGGV RTAALALLLG ALHWAPARCE EYDYYGWQAE PLHGRSYSKP PQCLDIPADL
61PLCHTVGYKR MRLPNLLEHE SLAEVKQQAS SWLPLLAKRC HSDTQVFLCS LFAPVCLDRP
121IYPCRSLCEA VRAGCAPLME AYGFPWPEML HCHKFPLDND LCIAVQFGHL PATAPPVTKI
181CAQCEMEHSA DGLMEQMCSS DFVVKMRIKE IKIENGDRKL IGAQKKKKLL KPGPLKRKDT
241KRLVLHMKNG AGCPCPQLDS LAGSFLVMGR KVDGQLLLMA VYRWDKKNKE MKFAVKFMFS
301YPCSLYYPFF YGAAEPH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

317 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.