Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SFRP-3 / FRZB Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99452-100
Catalog Number: LS-G99452
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FRZB (NM_001463), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FRZB | FRZB-1 | FIZ | FRE | FRITZ | Fritz and frizzled | Frizzled homolog-related | FRP-3 | FRZB-PEN | HFIZ | FRP | FZRB | SFRP-3 | SRFP3 | OS1 | Frezzled | Frizzled related protein 3 | Frizzled-related protein | Frizzled-related protein 1 | FRZB1 | SFRP3
- NCBI GenBank
- NM_001463
- SwissProt
- Q92765
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MVCGSPGGML LLRAGLLALA ALCLLRVPGA RAAACEPVRI PLCKSLPWNM TKMPNHLHHS61TQANAILAIE QFEGLLGTHC SPDLLFFLCA MYAPICTIDF QHEPIKPCKS VCERARQGCE121PILIKYRHSW PENLACEELP VYDRGVCISP EAIVTADGAD FPMDSSNGNC RGASSERCKC181KPIRATQKTY FRNNYNYVIR AKVKEIKTKC HDVTAVVEVK EILKSSLVNI PRDTVNLYTS241SGCLCPPLNV NEEYIIMGYE DEERSRLLLV EGSIAEKWKD RLGKKVKRWD MKLRHLGLSK301SDSSNSDSTQ SQKSGRNSNP RQARNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
325 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.