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SFMBT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SFMBT2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SFMBT2 antibodies →
$725.00each
Catalog Number: LS-G120805-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SFMBT2 (NM_001029880), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SFMBT2 | KIAA1617
Origin Species
Human
NCBI GenBank
R73354

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESTLSASNM QDPSSSPLEK CLGSANGNGD LDSEEGSSLE ETGFNWGEYL EETGASAAPH
61TSFKHVEISI QSNFQPGMKL EVANKNNPDT YWVATIITTC GQLLLLRYCG YGEDRRADFW
121CDVVIADLHP VGWCTQNNKV LMPPDAIKEK YTDWTEFLIR DLTGSRTAPA NLLEGPLRGK
181GPIDLITVGS LIELQDSQNP FQYWIVSVIE NVGGRLRLRY VGLEDTESYD QWLFYLDYRL
241RPVGWCQENK YRMDPPSEIY PLKMASEWKC TLEKSLIDAA KFPLPMEVFK DHADLRSHFF
301TVGMKLETVN MCEPFYISPA SVTKVFNNHF FQVTIDDLRP EPSKLSMLCH ADSLGILPVQ
361WCLKNGVSLT PPKGYSGQDF DWADYHKQHG AQEAPPFCFR NTSFSRGFTK NMKLEAVNPR
421NPGELCVASV VSVKGRLMWL HLEGLQTPVP EVIVDVESMD IFPVGWCEAN SYPLTAPHKT
481VSQKKRKIAV VQPEKQLPPT VPVKKIPHDL CLFPHLDTTG TVNGKYCCPQ LFINHRCFSG
541PYLNKGRIAE LPQSVGPGKC VLVLKEVLSM IINAAYKPGR VLRELQLVED PHWNFQEETL
601KAKYRGKTYR AVVKIVRTSD QVANFCRRVC AKLECCPNLF SPVLISENCP ENCSIHTKTK
661YTYYYGKRKK ISKPPIGESN PDSGHPKPAR RRKRRKSIFV QKKRRSSAVD FTAGSGEESE
721EEDADAMDDD TASEETGSEL RDDQTDTSSA EVPSARPRRA VTLRSGSEPV RRPPPERTRR
781GRGAPAASSA EEGEKCPPTK PEGTEDTKQE EEERLVLESN PLEWTVTDVV RFIKLTDCAP
841LAKIFQEQDI DGQALLLLTL PTVQECMELK LGPAIKLCHQ IERVKVAFYA QYAN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

894 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.