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SEZ6L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SEZ6L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SEZ6L antibodies →
$725.00each
Catalog Number: LS-G130355-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEZ6L (NM_001184773), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0927 | SEZ6L | Seizure 6-like protein | Seizure related gene 6-like
Origin Species
Human
NCBI GenBank
NM_021115
SwissProt
Q9BYH1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
112.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPAARPPAAG LRGISLFLAL LLGSPAAALE RDALPEGDAS PLGPYLLPSG APERGSPGKE
61HPEERVVTAP PSSSQSAEVL GELVLDGTAP SAHHDIPALS PLLPEEARPK HALPPKKKLP
121SLKQVNSARK QLRPKATSAA TVQRAGSQPA SQGLDLLSSS TEKPGPPGDP DPIVASEEAS
181EVPLWLDRKE SAVPTTPAPL QISPFTSQPY VAHTLPQRPE PGEPGPDMAQ EAPQEDTSPM
241ALMDKGENEL TGSASEESQE TTTSTIITTT VITTEQAPAL CSVSFSNPEG YIDSSDYPLL
301PLNNFLECTY NVTVYTGYGV ELQVKSVNLS DGELLSIRGV DGPTLTVLAN QTLLVEGQVI
361RSPTNTISVY FRTFQDDGLG TFQLHYQAFM LSCNFPRRPD SGDVTVMDLH SGGVAHFHCH
421LGYELQGAKI LTCINASKPH WSSQEPICSA PCGGAVHNAT IGRVLSPSYP ENTNGSQFCI
481WTIEAPEGQK LHLHFERLLL HDKDRMTVHS GQTNKSALLY DSLQTESVPF EGLLSEGNTI
541RIEFTSDQAR AASTFNIRFE AFEKGHCYEP YIQNGNFTTS DPTYNIGTIV EFTCDPGHSL
601EQGPAIIECI NVRDPYWNDT EPLCRAMCGG ELSAVAGVVL SPNWPEPYVE GEDCIWKIHV
661GEEKRIFLDI QFLNLSNSDI LTIYDGDEVM PHILGQYLGN SGPQKLYSST PDLTIQFHSD
721PAGLIFGKGQ GFIMNYIEVS RNDSCSDLPE IQNGWKTTSH TELVRGARIT YQCDPGYDIV
781GSDTLTCQWD LSWSSDPPFC EKIMYCTDPG EVDHSTRLIS DPVLLVGTTI QYTCNPGFVL
841EGSSLLTCYS RETGTPIWTS RLPHCVSEES LACDNPGLPE NGYQILYKRL YLPGESLTFM
901CYEGFELMGE VTIRCILGQP SHWNGPLPVC KVNQDSFEHA LEVAEAAAET SLEGGNMALA
961IFIPVLIISL LLGGAYIYIT RCRYYSNLRL PLMYSHPYSQ ITVETEFDNP IYETGETREY
1021EVSI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1024 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.