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SETDB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SETDB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SETDB2 antibodies →
$725.00each
Catalog Number: LS-G128769-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SETDB2(SETDB2) (NM_001160308), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C13orf4 | CLLD8 | CLLL8 | KMT1F | Lysine N-methyltransferase 1F | SETDB2 | SET domain bifurcated 2 | SET domain | bifurcated 2
Origin Species
Human
NCBI GenBank
NM_031915

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGEKNGDAKT FWMELEDDGK VDFIFEQVQN VLQSLKQKIK DGSATNKEYI QAMILVNEAT
61IINSSTSIKD PMPVTQKEQE NKSNAFPSTS CENSFPEDCT FLTTGNKEIL SLEDKVVDFR
121EKDSSSNLSY QSHDCSGACL MKMPLNLKGE NPLQLPIKCH FQRRHAKTNS HSSALHVSYK
181TPCGRSLRNV EEVFRYLLET ECNFLFTDNF SFNTYVQLAR NYPKQKEVVS DVDISNGVES
241VPISFCNEID SRKLPQFKYR KTVWPRAYNL TNFSSMFTDS CDCSEGCIDI TKCACLQLTA
301RNAKTSPLSS DKITTGYKYK RLQRQIPTGI YECSLLCKCN RQLCQNRVVQ HGPQVRLQVF
361KTEQKGWGVR CLDDIDRGTF VCIYSGRLLS RANTEKSYGI DENGRDENTM KNIFSKKRKL
421EVACSDCEVE VLPLGLETHP RTAKTEKCPP KFSNNPKELT METKYDNISR IQYHSVIRDP
481ESKTAIFQHN GKKMEFVSSE SVTPEDNDGF KPPREHLNSK TKGAQKDSSS NHVDEFEDNL
541LIESDVIDIT KYREETPPRS RCNQATTLDN QNIKKAIEVQ IQKPQEGRST ACQRQQVFCD
601EELLSETKNT SSDSLTKFNK GNVFLLDATK EGNVGRFLNH SCCPNLLVQN VFVETHNRNF
661PLVAFFTNRY VKARTELTWD YGYEAGTVPE KEIFCQCGVN KCRKKIL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

707 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.