Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SETD8 / SET8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101662-100
Catalog Number: LS-G101662
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human KMT5A(KMT5A) (NM_020382), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KMT5A | Lysine N-methyltransferase 5A | PR/SET07 | SETD8 | PRSET7 | SET8 | H4-K20-HMTase SETD8 | PR-Set7 | SET07
- Origin Species
- Human
- NCBI GenBank
- NM_020382
- SwissProt
- Q9NQR1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARGRKMSKP RAVEAAAAAA AVAATAPGPE MVERRGPGRP RTDGENVFTG QSKIYSYMSP61NKCSGMRFPL QEENSVTHHE VKCQGKPLAG IYRKREEKRN AGNAVRSAMK SEEQKIKDAR121KGPLVPFPNQ KSEAAEPPKT PPSSCDSTNA AIAKQALKKP IKGKQAPRKK AQGKTQQNRK181LTDFYPVRRS SRKSKAELQS EERKRIDELI ESGKEEGMKI DLIDGKGRGV IATKQFSRGD241FVVEYHGDLI EITDAKKREA LYAQDPSTGC YMYYFQYLSK TYCVDATRET NRLGRLINHS301KCGNCQTKLH DIDGVRHLIL IASRDIAAGE ELLYDYGDRS KASIEAHPWL KHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
352 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.