Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SETD1A / SET1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113714-100
Catalog Number: LS-G113714
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human hSET1(SETD1A) (NM_014712), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HSET1A | KIAA0339 | Lysine N-methyltransferase 2F | KMT2F | Set1 | SET domain containing 1A | Set1A | SETD1A
- Origin Species
- Human
- NCBI GenBank
- AB002337
- SwissProt
- O15047
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 185.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDQEGGGDGQ KAPSFQWRNY KLIVDPALDP ALRRPSQKVY RYDGVHFSVN DSKYIPVEDL61QDPRCHVRSK NRDFSLPVPK FKLDEFYIGQ IPLKEVTFAR LNDNVRETFL KDMCRKYGEV121EEVEILLHPR TRKHLGLARV LFTSTRGAKE TVKNLHLTSV MGNIIHAQLD IKGQQRMKYY181ELIVNGSYTP QTVPTGGKAL SEKFQGSGAA TETAESRRRS SSDTAAYPAG TTAVGTPGNG241TPCSQDTSFS SSRQDTPSSF GQFTPQSSQG TPYTSRGSTP YSQDSAYSSS TTSTSFKPRR301SENSYQDAFS RRHFSASSAS TTASTAIAAT TAATASSSAS SSSLSSSSSS SSSSSSSQFR361SSDANYPAYY ESWNRYQRHT SYPPRRATRE EPPGAPFAEN TAERFPPSYT SYLPPEPSRP421TDQDYRPPAS EAPPPEPPEP GGGGGGGGPS PEREEVRTSP RPASPARSGS PAPETTNESV481PFAQHSSLDS RIEMLLKEQR SKFSFLASDT EEEEENSSMV LGARDTGSEV PSGSGHGPCT541PPPAPANFED VAPTGSGEPG ATRESPKANG QNQASPCSSG DDMEISDDDR GGSPPPAPTP601PQQPPPPPPP PPPPPPYLAS LPLGYPPHQP AYLLPPRPDG PPPPEYPPPP PPPPHIYDFV661NSLELMDRLG AQWGGMPMSF QMQTQMLTRL HQLRQGKGLI AASAGPPGGA FGEAFLPFPP721PQEAAYGLPY ALYAQGQEGR GAYSREAYHL PMPMAAEPLP SSSVSGEEAR LPPREEAELA781EGKTLPTAGT VGRVLAMLVQ EMKSIMQRDL NRKMVENVAF GAFDQWWESK EEKAKPFQNA841AKQQAKEEDK EKTKLKEPGL LSLVDWAKSG GTTGIEAFAF GSGLRGALRL PSFKVKRKEP901SEISEASEEK RPRPSTPAEE DEDDPEQEKE AGEPGRPGTK PPKRDEERGK TQGKHRKSFA961LDSEGEEASQ ESSSEKDEED DEEDEEDEDR EEAVDTTKKE TEVSDGEDEE SDSSSKCSLY1021ADSDGENDST SDSESSSSSS SSSSSSSSSS SSSSSSSSES SSEDEEEEER PAALPSASPP1081PREVPVPTPA PVEVPVPERV AGSPVTPLPE QEASPARPAG PTEESPPSAP LRPPEPPAGP1141PAPAPRPDER PSSPIPLLPP PKKRRKTVSF SAIEVVPAPE PPPATPPQAK FPGPASRKAP1201RGVERTIRNL PLDHASLVKS WPEEVSRGGR SRAGGRGRLT EEEEAEPGTE VDLAVLADLA1261LTPARRGLPA LPAVEDSEAT ETSDEAERPR PLLSHILLEH NYALAVKPTP PAPALRPPEP1321VPAPAALFSS PADEVLEAPE VVVAEAEEPK PQQLQQQREE GEEEGEEEGE EEEEESSDSS1381SSSDGEGALR RRSLRSHARR RRPPPPPPPP PPRAYEPRSE FEQMTILYDI WNSGLDSEDM1441SYLRLTYERL LQQTSGADWL NDTHWVHHTI TNLTTPKRKR RPQDGPREHQ TGSARSEGYY1501PISKKEKDKY LDVCPVSARQ LEGVDTQGTN RVLSERRSEQ RRLLSAIGTS AIMDSDLLKL1561NQLKFRKKKL RFGRSRIHEW GLFAMEPIAA DEMVIEYVGQ NIRQMVADMR EKRYVQEGIG1621SSYLFRVDHD TIIDATKCGN LARFINHCCT PNCYAKVITI ESQKKIVIYS KQPIGVDEEI1681TYDYKFPLED NKIPCLCGTE SCRGSLNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1707 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.