Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SERPINI2 / PANCPIN Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115459-100
Catalog Number: LS-G115459
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SERPINI2 (NM_006217), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Peptidase inhibitor 14 | PI-14 | PI14 | SERPIN12 | SERPINI2 | TSA2004 | MEPI | PANCPIN | Protease inhibitor 14 | Serpin I2
- Origin Species
- Human
- NCBI GenBank
- NM_006217
- SwissProt
- O75830
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.39 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIQNLMREVK MDTIFLWSLL LLFFGSQASR CSAQKNTEFA VDLYQEVSLS HKDNIIFSPL61GITLVLEMVQ LGAKGKAQQQ IRQTLKQQET SAGEEFFVLK SFFSAISEKK QEFTFNLANA121LYLQEGFTVK EQYLHGNKEF FQSAIKLVDF QDAKACAEMI STWVERKTDG KIKDMFSGEE181FGPLTRLVLV NAIYFKGDWK QKFRKEDTQL INFTKKNGST VKIPMMKALL RTKYGYFSES241SLNYQVLELS YKGDEFSLII ILPAEGMDIE EVEKLITAQQ ILKWLSEMQE EEVEISLPRF301KVEQKVDFKD VLYSLNITEI FSGGCDLSGI TDSSEVYVSQ VTQKVFFEIN EDGSEAATST361GIHIPVIMSL AQSQFIANHP FLFIMKHNPT ESILFMGRVT NPDTQEIKGR DLDSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
415 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.