Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SERPINH1 / HSP47 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99380-100
Catalog Number: LS-G99380
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Hsp47(SERPINH1) (NM_001235), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 47 kDa heat shock protein | AsTP3 | Colligin | Colligin-1 | Collagen-binding protein | Colligin-2 | CBP1 | HSP47 | OI10 | Serpin H1 | SERPINH1 | SERPINH2 | CBP2 | Collagen-binding protein 1 | Colligen | gp46 | PIG14 | PPROM | RA-A47
- NCBI GenBank
- NM_001235
- SwissProt
- P50454
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.47 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRSLLLLSAF CLLEAALAAE VKKPAAAAAP GTAEKLSPKA ATLAERSAGL AFSLYQAMAK61DQAVENILVS PVVVASSLGL VSLGGKATTA SQAKAVLSAE QLRDEEVHAG LGELLRSLSN121STARNVTWKL GSRLYGPSSV SFADDFVRSS KQHYNCEHSK INFRDKRSAL QSINEWAAQT181TDGKLPEVTK DVERTDGALL VNAMFFKPHW DEKFHHKMVD NRGFMVTRSY TVGVMMMHRT241GLYNYYDDEK EKLQIVEMPL AHKLSSLIIF MPHHVEPLER LEKLLTKEQL KIWMGKMQKK301AVAISLPKGV VEVTHDLQKH LAGLGLTEAI DKNKADLSRM SGKKDLYLAS VFHATAFELD361TDGNPFDQDI YGREELRSPK LFYADHPFIF LVRDTQSGSL LFIGRLVRPK GDKMRDELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
418 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.