Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SERPINB13 / HUR7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114458-100
Catalog Number: LS-G114458
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SERPINB13 (NM_012397), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HaCaT UV-repressible serpin | HSHUR7SEQ | HUR7 | Peptidase inhibitor 13 | PI-13 | SERPINB13 | Serpin B13 | UV-B repressed sequence | HUR 7 | Headpin | HURPIN | PI13 | Proteinase inhibitor 13
- Origin Species
- Human
- NCBI GenBank
- NM_012397
- SwissProt
- Q9UIV8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSLGAVSTR LGFDLFKELK KTNDGNIFFS PVGILTAIGM VLLGTRGATA SQLEEVFHSE61KETKSSRIKA EEKEVIENTE AVHQQFQKFL TEISKLTNDY ELNITNRLFG EKTYLFLQKY121LDYVEKYYHA SLEPVDFVNA ADESRKKINS WVESKTNEKI KDLFPDGSIS SSTKLVLVNM181VYFKGQWDRE FKKENTKEEK FWMNKSTSKS VQMMTQSHSF SFTFLEDLQA KILGIPYKNN241DLSMFVLLPN DIDGLEKIID KISPEKLVEW TSPGHMEERK VNLHLPRFEV EDGYDLEAVL301AAMGMGDAFS EHKADYSGMS SGSGLYAQKF LHSSFVAVTE EGTEAAAATG IGFTVTSAPG361HENVHCNHPF LFFIRHNESN SILFFGRFSS PTRTRPLEQKLISEEDLAANDILDYKDDDDKV
391 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.