Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SERPINB11 / EPIPIN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107882-100
Catalog Number: LS-G107882
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SERPINB11 (NM_080475), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SERPINB11 | Serpin B11 | EPIPIN | SERPIN11
- Origin Species
- Human
- SwissProt
- Q96P15
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSLSTANVE FCLDVFKELN SNNIGDNIFF SSLSLLYALS MVLLGARGET AEQLEKVLHF61SHTVDSLKPG FKDSPKCSQA GRIHSEFGVE FSQINQPDSN CTLSIANRLY GTKTMAFHQQ121YLSCSEKWYQ ARLQTVDFEQ STEETRKMIN AWVENKTNGK VANLFGKSTI DPSSVMVLVN181TIYFKGQRQN KFQVRETVKS PFQLSEGKNV TVEMMYQIGT FKLAFVKEPQ MQVLELPYVN241NKLSMIILLP VGIANLKQIE KQLNSGTFHE WTSSSNMMER EVEVHLPRFK LEIKYELNSL301LKPLGVTDLF NQVKADLSGM SPTKGLYLSK AIHKSYLDVS EEGTEAAAAT GDSIAVKSLP361MRAQFKANHP FLFFIRHTHT NTILFCGKLA SPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
392 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.