Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SERPINA1 / Alpha 1 Antitrypsin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G98997-100
Catalog Number: LS-G98997
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human alpha 1 Antitrypsin(SERPINA1) (NM_000295), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A1A | A1AT | AAT | Alpha 1 Antitrypsin | Alpha-1 protease inhibitor | Alpha-1-antiproteinase | Alpha-1-antitrypsin | Alpha1AT | PRO2275 | Alpha-1-antitrypsin null | SERPINA1 | PI1 | Serpin A1 | Alpha 1 AT
- NCBI GenBank
- K02212
- SwissProt
- P01009
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.72 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSSVSWGIL LLAGLCCLVP VSLAEDPQGD AAQKTDTSHH DQDHPTFNKI TPNLAEFAFS61LYRQLAHQSN STNIFFSPVS IATAFAMLSL GTKADTHDEI LEGLNFNLTE IPEAQIHEGF121QELLRTLNQP DSQLQLTTGN GLFLSEGLKL VDKFLEDVKK LYHSEAFTVN FGDTEEAKKQ181INDYVEKGTQ GKIVDLVKEL DRDTVFALVN YIFFKGKWER PFEVKDTEEE DFHVDQVTTV241KVPMMKRLGM FNIQHCKKLS SWVLLMKYLG NATAIFFLPD EGKLQHLENE LTHDIITKFL301ENEDRRSASL HLPKLSITGT YDLKSVLGQL GITKVFSNGA DLSGVTEEAP LKLSKAVHKA361VLTIDEKGTE AAGAMFLEAI PMSIPPEVKF NKPFVFLMID QNTKSPLFMG KVVNPTQKTR421TRPLEQKLIS EEDLAANDIL DYKDDDDKV
449 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.