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SERINC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SERINC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G103425-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SERINC3 (NM_198941), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AIGP1 | DIFF33 | SERINC3 | TDE1 | SBBI99 | Serine incorporator 3 | TMS-1 | Transmembrane protein SBBI99 | TDE
Origin Species
Human
NCBI GenBank
NM_006811
SwissProt
Q13530

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MGAVLGVFSL ASWVPCLCSG ASCLLCSCCP NSKNSTVTRL IYAFILLLST VVSYIMQRKE
61METYLKKIPG FCEGGFKIHE ADINADKDCD VLVGYKAVYR ISFAMAIFFF VFSLLMFKVK
121TSKDLRAAVH NGFWFFKIAA LIGIMVGSFY IPGGYFSSVW FVVGMIGAAL FILIQLVLLV
181DFAHSWNESW VNRMEEGNPR LWYAALLSFT SAFYILSIIC VGLLYTYYTK PDGCTENKFF
241ISINLILCVV ASIISIHPKI QEHQPRSGLL QSSLITLYTM YLTWSAMSNE PDRSCNPNLM
301SFITRITAPT LAPGNSTAVV PTPTPPSKSG SLLDSDNFIG LFVFVLCLLY SSIRTSTNSQ
361VDKLTLSGSD SVILGDTTTS GASDEEDGQP RRAVDNEKEG VQYSYSLFHL MLCLASLYIM
421MTLTSWYSPD AKFQSMTSKW PAVWVKISSS WVCLLLYVWT LVAPLVLTSR DFS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

473 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.