Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEPT9 / Septin 9 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G124292-100
Catalog Number: LS-G124292
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MSF(SEPT9) (NM_001113493), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AF17q25 | Ov/Br septin | PNUTL4 | KIAA0991 | SEPT9 | Septin 9 | Septin D1 | MLL septin-like fusion | MLL septin-like fusion protein | SINT1 | MSF | MSF1 | Ovarian/Breast septin | SeptD1 | Septin-9
- Origin Species
- Human
- NCBI GenBank
- NM_006640
- SwissProt
- Q9UHD8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSDPAVNAQL DGIISDFEAL KRSFEVEEVE TPNSTPPRRV QTPLLRATVA SSTQKFQDLG61VKNSEPSARH VDSLSQRSPK ASLRRVELSG PKAAEPVSRR TELSIDISSK QVENAGAIGP121SRFGLKRAEV LGHKTPEPAP RRTEITIVKP QESAHRRMEP PASKVPEVPT APATDAAPKR181VEIQMPKPAE APTAPSPAQT LENSEPAPVS QLQSRLEPKP QPPVAEATPR SQEATEAAPS241CVGDMADTPR DAGLKQAPAS RNEKAPVDFG YVGIDSILEQ MRRKAMKQGF EFNIMVVGQS301GLGKSTLINT LFKSKISRKS VQPTSEERIP KTIEIKSITH DIEEKGVRMK LTVIDTPGFG361DHINNENCWQ PIMKFINDQY EKYLQEEVNI NRKKRIPDTR VHCCLYFIPA TGHSLRPLDI421EFMKRLSKVV NIVPVIAKAD TLTLEERVHF KQRITADLLS NGIDVYPQKE FDEDSEDRLV481NEKFREMIPF AVVGSDHEYQ VNGKRILGRK TKWGTIEVEN TTHCEFAYLR DLLIRTHMQN541IKDITSSIHF EAYRVKRLNE GSSAMANGME EKEPEAPEMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
579 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.