Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEPT7 / Septin 7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118398-100
Catalog Number: LS-G118398
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEPT7 (NM_001788), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDC10 | CDC10 protein homolog | Hcdc10 | NBLA02942 | Sep7 | SEPT7A | Septin 7 | SEPT7 | Septin-7 | CDC3
- Origin Species
- Human
- NCBI GenBank
- NM_001788
- SwissProt
- Q16181
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.66 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQQKNLEGY VGFANLPNQV YRKSVKRGFE FTLMVVGESG LGKSTLINSL FLTDLYSPEY61PGPSHRIKKT VQVEQSKVLI KEGGVQLLLT IVDTPGFGDA VDNSNCWQPV IDYIDSKFED121YLNAESRVNR RQMPDNRVQC CLYFIAPSGH GLKPLDIEFM KRLHEKVNII PLIAKADTLT181PEECQQFKKQ IMKEIQEHKI KIYEFPETDD EEENKLVKKI KDRLPLAVVG SNTIIEVNGK241RVRGRQYPWG VAEVENGEHC DFTILRNMLI RTHMQDLKDV TNNVHYENYR SRKLAAVTYN301GVDNNKNKGQ LTKSPLAQME EERREHVAKM KKMEMEMEQV FEMKVKEKVQ KLKDSEAELQ361RRHEQMKKNL EAQHKELEEK RRQFEDEKAN WEAQQRILEQ QNSSRTLEKN KKKGKIFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
417 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.