Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEPT4 / Septin 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102191-100
Catalog Number: LS-G102191
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEPT4 (NM_080415), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Brain protein H5 | ARTS | BRADEION | CE5B3 | Cerebral protein 7 | H5 | HCDCREL-2 | Peanut-like 2 (Drosophila) | PNUTL2 | SEP4 | SEPT4 | Septin 4 | Bradeion beta | CE5B3 beta | Hucep-7 | MART | Peanut-like protein 2 | Septin-4 | Septin-M
- Origin Species
- Human
- NCBI GenBank
- NM_004574
- SwissProt
- O43236
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 30.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIKRFLEDTT DDGELSKFVK DFSGNASCHP PEAKTWASRP QVPEPRPQAP DLYDDDLEFR61PPSRPQSSDN QQYFCAPAPL SPSARPRSPW GKLDPYDSSE DDKEYVGFAT LPNQVHRKSV121KKGFDFTLMV AGESGLGKST LVNSLFLTDL YRDRKLLGAE ERIMQTVEIT KHAVDIEEKG181VRLRLTIVDT PGFGDAVNNT ECWKPVAEYI DQQFEQYFRD ESGLNRKNIQ DNRVHCCLYF241ISPFGHGYGP SLRLLAPPGA VKGTGQEHQG QGCHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
274 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.