LSBio
SEPT10 /Septin 10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SEPT10 /Septin 10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104613-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEPT10 (NM_178584), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Septin-10 | SEPT10 | Septin 10
Origin Species
Hu
NCBI GenBank
AF146760
SwissProt
Q9P0V9

Protein

Expression System
HEK293
Fusion Tag
FLAG, Myc
Molecular Weight
49.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASSEVARHL KRENIRSLTM SGHVGFESLP DQLVNRSIQQ GFCFNILCVG ETGIGKSTLI
61DTLFNTNFED YESSHFCPNV KLKAQTYELQ ESNVQLKLTI VNTVGFGDQI NKEESYQPIV
121DYIDAQFEAY LQEELKIKRS LFTYHDSRIH VCLYFISPTG HSLKTLDLLT MKNLDSKVNI
181IPVIAKADTV SKTELQKFKI KLMSELVSNG VQIYQFPTDD DTIAKVNAAM NGQLPFAVVG
241SMDEVKVGNK MVKARQYPWG VVQVENENHC DFVKLREMLI CTNMEDLREQ THTRHYELYR
301RCKLEEMGFT DVGPENKPVS VQETYEAKRH EFHGERQRKE EEMKQMFVQR VKEKEAILKE
361AERELQAKFE HLKRLHQEER MKLEEKRRLL EEEIIAFSKK KATSEIFHSQ SFLATGSNLR
421KDKDRKNSNF L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

431 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.