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SENP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SENP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111000-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SENP7 (NM_020654), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1707 | Sentrin-specific protease 7 | SUMO-1-specific protease 2 | SUMO-1 protease-2 | SUSP2 | SENP7 | SSP2
Origin Species
Human
NCBI GenBank
NM_020654
SwissProt
Q9BQF6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
119.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDKRKLGRRP SSSEIITEGK RKKSSSDLSE IRKMLNAKPE DVHVQSPLSK FRSSERWTLP
61LQWERSLRNK VISLDHKNQK HIRGCPVTSK SSPERQLKVM LTNVLWTDLG RKFRKTLPRN
121DANLCDANKV QSDSLPSTSV DSLETCQKLE PLRQSLNLSE RIPRVILTNV LGTELGRKYI
181RTPPVTEGSL SDTDNLQSEQ LSSSSDGSLE SYQNLNPHKS CYLSERGSQR SKTVDDNSAK
241QTAHNKEKRR KDDGISLLIS DTQPEDLNSG SRGCDHLEQE SRNKDVKYSD SKVELTLISR
301KTKRRLRNNL PDSQYCTSLD KSTEQTKKQE DDSTISTEFE KPSENYHQDP KLPEEITTKP
361TKSDFTKLSS LNSQELTLSN ATKSASAGST TETVENSNSI DIVGISSLVE KDENELNTIE
421KPILRGHNEG NQSLISAEPI VVSSDEEGPV EHKSSEILKL QSKQDRETTN ENESTSESAL
481LELPLITCES VQMSSELCPY NPVMENISSI MPSNEMDLQL DFIFTSVYIG KIKGASKGCV
541TITKKYIKIP FQVSLNEISL LVDTTHLKRF GLWKSKDDNH SKRSHAILFF WVSSDYLQEI
601QTQLEHSVLS QQSKSSEFIF LELHNPVSQR EELKLKDIMT EISIISGELE LSYPLSWVQA
661FPLFQNLSSK ESSFIHYYCV STCSFPAGVA VAEEMKLKSV SQPSNTDAAK PTYTFLQKQS
721SGCYSLSITS NPDEEWREVR HTGLVQKLIV YPPPPTKGGL GVTNEDLECL EEGEFLNDVI
781IDFYLKYLIL EKASDELVER SHIFSSFFYK CLTRKENNLT EDNPNLSMAQ RRHKRVRTWT
841RHINIFNKDY IFVPVNESSH WYLAVICFPW LEEAVYEDFP QTVSQQSQAQ QSQNDNKTID
901NDLRTTSTLS LSAEDSQSTE SNMSVPKKMC KRPCILILDS LKAASVQNTV QNLREYLEVE
961WEVKLKTHRQ FSKTNMVDLC PKVPKQDNSS DCGVYLLQYV ESFFKDPIVN FELPIHLEKW
1021FPRHVIKTKR EDIRELILKL HLQQQKGSSS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1050 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.