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SENP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SENP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102368-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SENP5 (NM_152699), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SENP5 | Sentrin-specific protease 5 | FKSG45
Origin Species
Human
NCBI GenBank
NM_152699
SwissProt
Q96HI0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKKQRKILWR KGIHLAFSEK WNTGFGGFKK FYFHQHLCIL KAKLGRPVTW NRQLRHFQGR
61KKALQIQKTW IKDEHLCAKT KFNVATQNVS TLSSKVKRKD AKHFISSSKT LLRLQAEKLL
121SSAKNSDHEY CREKNLLKAV TDFPSNSALG QANGHRPRTD PQPSDFPMKF NGESQSPGES
181GTIVVTLNNH KRKGFCYGCC QGPEHHRNGG PLIPKKFQLN QHRRIKLSPL MMYEKLSMIR
241FRYRILRSQH FRTKSKVCKL RKAQRSWVQK VTGDHQETRR ENGEGGSCSP FPSPEPKDPS
301CRHQPYFPDM DSSAVVKGTN SHVPDCHTKG SSFLGKELSL DEAFPDQQNG SATNAWDQSS
361CSSPKWECTE LIHDIPLPEH RSNTMFISET EREIMTLGQE NQTSSVSDDR VKLSVSGADT
421SVSSVDGPVS QKAVQNENSY QMEEDGSLKQ SILSSELLDH PYCKSPLEAP LVCSGLKLEN
481QVGGGKNSQK ASPVDDEQLS VCLSGFLDEV MKKYGSLVPL SEKEVLGRLK DVFNEDFSNR
541KPFINREITN YRARHQKCNF RIFYNKHMLD MDDLATLDGQ NWLNDQVINM YGELIMDAVP
601DKVHFFNSFF HRQLVTKGYN GVKRWTKKVD LFKKSLLLIP IHLEVHWSLI TVTLSNRIIS
661FYDSQGIHFK FCVENIRKYL LTEAREKNRP EFLQGWQTAV TKCIPQQKND SDCGVFVLQY
721CKCLALEQPF QFSQEDMPRV RKRIYKELCE CRLMD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

755 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.