Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEMA6C / Semaphorin 6C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G130327-100
Catalog Number: LS-G130327
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEMA6C (NM_001178061), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1869 | M-SemaY2 | SEMA6C | Semaphorin-6C | Semaphorin-Y | M-SemaY | Sema Y | SEMAY
- Origin Species
- Human
- NCBI GenBank
- NM_030913
- SwissProt
- Q9H3T2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 103.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPRAPHFMPL LLLLLLLSLP HTQAAFPQDP LPLLISDLQG TSPLSWFRGL EDDAVAAELG61LDFQRFLTLN RTLLVAARDH VFSFDLQAEE EGEGLVPNKY LTWRSQDVEN CAVRGKLTDE121CYNYIRVLVP WDSQTLLACG TNSFSPVCRS YGITSLQQEG EELSGQARCP FDATQSNVAI181FAEGSLYSAT AADFQASDAV VYRSLGPQPP LRSAKYDSKW LREPHFVQAL EHGDHVYFFF241REVSVEDARL GRVQFSRVAR VCKRDMGGSP RALDRHWTSF LKLRLNCSVP GDSTFYFDVL301QALTGPVNLH GRSALFGVFT TQTNSIPGSA VCAFYLDEIE RGFEGKFKEQ RSLDGAWTPV361SEDRVPSPRP GSCAGVGGAA LFSSSRDLPD DVLTFIKAHP LLDPAVPPVT HQPLLTLTSR421ALLTQVAVDG MAGPHSNITV MFLGSNDGTV LKVLTPGGRS GGPEPILLEE IDAYSPARCS481GKRTAQTARR IIGLELDTEG HRLFVAFSGC IVYLPLSRCA RHGACQRSCL ASQDPYCGWH541SSRGCVDIRG SGGTDVDQAG NQESMEHGDC QDGATGSQSG PGDSAYVLPG PGPSPGTPSP601PSDAHPRPQS STLGVHTRGV RRDLPPASAS RSVPIPLLLA SVAAAFALGA SVSGLLVSCA661CRRAHRRRGK DIETPGLPRP LSLRSLARLH GGGPEPPPPS KDGDAVQTPQ LYTTFLPPPE721GVPPPELACL PTPESTPELP VKHLRAAGDP WEWNQNRNNA KEGPGRSRGG HAAGGPAPRV781LVRPPPPGCP GQAVEVTTLE ELLRYLHGPQ PPRKGAEPPA PLTSRALPPE PAPALLGGPS841PRPHECASPL RLDVPPEGRC ASAPARPALS APAPRLGVGG GRRLPFSGHR APPALLTRVP901SGGPSRYSGG PGKHLLYLGR PEGYRGRALK RVDVEKPQLS LKPPLVGPSS RQAVPNGGRF961NFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
962 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.