Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEMA4G / Semaphorin 4G Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112150-100
Catalog Number: LS-G112150
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEMA4G (NM_017893), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1619 | SEMA4G | Semaphorin-4G
- Origin Species
- Human
- NCBI GenBank
- NM_017893
- SwissProt
- Q9NTN9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWGRLWPLLL SILTATAVPG PSLRRPSREL DATPRMTIPY EELSGTRHFK GQAQNYSTLL61LEEASARLLV GARGALFSLS ANDIGDGAHK EIHWEASPEM QSKCHQKGKN NQTECFNHVR121FLQRLNSTHL YACGTHAFQP LCAAIDAEAF TLPTSFEEGK EKCPYDPARG FTGLIIDGGL181YTATRYEFRS IPDIRRSRHP HSLRTEETPM HWLNDAEFVF SVLVRESKAS AVGDDDKVYY241FFTERATEEG SGSFTQSRSS HRVARVARVC KGDLGGKKIL QKKWTSFLKA RLICHIPLYE301TLRGVCSLDA ETSSRTHFYA AFTLSTQWKT LEASAICRYD LAEIQAVFAG PYMEYQDGSR361RWGRYEGGVP EPRPGSCITD SLRSQGYNSS QDLPSLVLDF VKLHPLMARP VVPTRGRPLL421LKRNIRYTHL TGTPVTTPAG PTYDLLFLGT ADGWIHKAVV LGSGMHIIEE TQVFRESQSV481ENLVISLLQH SLYVGAPSGV IQLPLSSCSR YRSCYDCILA RDPYCGWDPG THACAAATTI541ANRSQGSRTA LIQDIERGNR GCESSRDTGP PPPLKTRSVL RGDDVLLPCD QPSNLARALW601LLNGSMGLSD GQGGYRVGVD GLLVTDAQPE HSGNYGCYAE ENGLRTLLAS YSLTVRPATP661APAPKAPATP GAQLAPDVRL LYVLAIAALG GLCLILASSL LYVACLREGR RGRRRKYSLG721RASRAGGSAV QLQTVSGQCP GEEDEGDDEG AGGLEGSCLQ IIPGEGAPAP PPPPPPPPPA781ELTNGLVALP SRLRRMNGNS YVLLRQSNNG VPAGPCSFAE ELSRILEKRK HTQLVEQLDE841SSVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
843 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.