Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEMA4F / Semaphorin 4F Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116764-100
Catalog Number: LS-G116764
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEMA4F (NM_004263), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- M-SEMA | S4F | Sema M | Semaphorin M | Semaphorin-4F | Semaphorin-W | SEMAW | PRO2353 | Sema W | SEMAM | M-Sema-M | SEMA4F | Semaphorin-M
- Origin Species
- Human
- NCBI GenBank
- NM_004263
- SwissProt
- O95754
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 83.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPASAARPRP GPGQPTASPF PLLLLAVLSG PVSGRVPRSV PRTSLPISEA DSCLTRFAVP61HTYNYSVLLV DPASHTLYVG ARDTIFALSL PFSGERPRRI DWMVPEAHRQ NCRKKGKKED121ECHNFVQILA IANASHLLTC GTFAFDPKCG VIDVSRFQQV ERLESGRGKC PFEPAQRSAA181VMAGGVLYAA TVKNYLGTEP IITRAVGRAE DWIRTDTLPS WLNAPAFVAA VALSPAEWGD241EDGDDEIYFF FTETSRAFDS YERIKVPRVA RVCAGDLGGR KTLQQRWTTF LKADLLCPGP301EHGRASSVLQ DVAVLRPELG AGTPIFYGIF SSQWEGATIS AVCAFRPQDI RTVLNGPFRE361LKHDCNRGLP VVDNDVPQPR PGECITNNMK LRHFGSSLSL PDRVLTFIRD HPLMDRPVFP421ADGHPLLVTT DTAYLRVVAH RVTSLSGKEY DVLYLGTEDG HLHRAVRIGA QLSVLEDLAL481FPEPQPVENM KLYHSWLLVG SRTEVTQVNT TNCGRLQSCS ECILAQDPVC AWSFRLDECV541AHAGEHRGLV QDIESADVSS LCPKEPGERP VVFEVPVATA AHVVLPCSPS SAWASCVWHQ601PSGVTALTPR RDGLEVVVTP GAMGAYACEC QEGGAAHVVA AYSLVWGSQR DAPSRAHTVG661AGLAGFFLGI LAASLTLILI GRRQQRRRQR ELLARDKVGL DLGAPPSGTT SYSQDPPSPS721PEDERLPLAL AKRGSGFGGF SPPFLLDPCP SPAHIRLTGA PLATCDETSITRTRPLEQKLISEEDLAANDILDYKDDDDKV
770 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.