Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEMA3F / Semaphorin 3F Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116814-100
Catalog Number: LS-G116814
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Semaphorin 3F(SEMA3F) (NM_004186), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SEMA3F | Semaphorin III/F | Semaphorin IV | Semaphorin-3F | Sema III/F | Sema IV | SEMA-IV | SEMA4 | SEMAK
- Origin Species
- Human
- NCBI GenBank
- NM_004186
- SwissProt
- Q13275
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLVAGLLLWA SLLTGAWPSF PTQDHLPATP RVRLSFKELK ATGTAHFFNF LLNTTDYRIL61LKDEDHDRMY VGSKDYVLSL DLHDINREPL IIHWAASPQR IEECVLSGKD VNGECGNFVR121LIQPWNRTHL YVCGTGAYNP MCTYVNRGRR AQATPWTQTQ AVRGRGSRAT DGALRPMPTA181PRQDYIFYLE PERLESGKGK CPYDPKLDTA SALINEELYA GVYIDFMGTD AAIFRTLGKQ241TAMRTDQYNS RWLNDPSFIH AELIPDSAER NDDKLYFFFR ERSAEAPQSP AVYARIGRIC301LNDDGGHCCL VNKWSTFLKA RLVCSVPGED GIETHFDELQ DVFVQQTQDV RNPVIYAVFT361SSGSVFRGSA VCVYSMADIR MVFNGPFAHK EGPNYQWMPF SGKMPYPRPG TCPGGTFTPS421MKSTKDYPDE VINFMRSHPL MYQAVYPLQR RPLVVRTGAP YRLTTIAVDQ VDAADGRYEV481LFLGTDRGTV QKVIVLPKDD QEMEELMLEE VEVFKDPAPV KTMTISSKRQ QLYVASAVGV541THLSLHRCQA YGAACADCCL ARDPYCAWDG QACSRYTASS KRRSRRQDVR HGNPIRQCRG601FNSNANKNAV ESVQYGVAGS AAFLECQPRS PQATVKWLFQ RDPGDRRREI RAEDRFLRTE661QGLLLRALQL SDRGLYSCTA TENNFKHVVT RVQLHVLGRD AVHAALFPPL SMSAPPPPGA721GPPTPPYQEL AQLLAQPEVG LIHQYCQGYW RHVPPSPREA PGAPRSPEPQ DQKKPRNRRH781HPPDTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
785 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.