Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEMA3C / Semaphorin 3C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100799-100
Catalog Number: LS-G100799
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Semaphorin 3c(SEMA3C) (NM_006379), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Semaphorin-E | SEMAE | SemE | SEMA3C | Semaphorin-3C | Sema E | Semaphorin E
- Origin Species
- Human
- NCBI GenBank
- NM_006379
- SwissProt
- Q99985
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 85 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAFRTICVLV GVFICSICVK GSSQPQARVY LTFDELRETK TSEYFSLSHH PLDYRILLMD61EDQDRIYVGS KDHILSLNIN NISQEALSVF WPASTIKVEE CKMAGKDPTH GCGNFVRVIQ121TFNRTHLYVC GSGAFSPVCT YLNRGRRSED QVFMIDSKCE SGKGRCSFNP NVNTVSVMIN181EELFSGMYID FMGTDAAIFR SLTKRNAVRT DQHNSKWLSE PMFVDAHVIP DGTDPNDAKV241YFFFKEKLTD NNRSTKQIHS MIARICPNDT GGLRSLVNKW TTFLKARLVC SVTDEDGPET301HFDELEDVFL LETDNPRTTL VYGIFTTSSS VFKGSAVCVY HLSDIQTVFN GPFAHKEGPN361HQLISYQGRI PYPRPGTCPG GAFTPNMRTT KEFPDDVVTF IRNHPLMYNS IYPIHKRPLI421VRIGTDYKYT KIAVDRVNAA DGRYHVLFLG TDRGTVQKVV VLPTNNSVSG ELILEELEVF481KNHAPITTMK ISSKKQQLYV SSNEGVSQVS LHRCHIYGTA CADCCLARDP YCAWDGHSCS541RFYPTGKRRS RRQDVRHGNP LTQCRGFNLK AYRNAAEIVQ YGVKNNTTFL ECAPKSPQAS601IKWLLQKDKD RRKEVKLNER IIATSQGLLI RSVQGSDQGL YHCIATENSF KQTIAKINFK661VLDSEMVAVV TDKWSPWTWA SSVRALPFHP KDIMGAFSHS EMQMINQYCK DTRQQHQQGD721ESQKMRGDYG KLKALINSRK SRNRRNQLPE STRTRPLEQKLISEEDLAANDILDYKDDDDKV
751 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.