Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SELP / P-Selectin / CD62P Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117615-100
Catalog Number: LS-G117615
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CD62P(SELP) (NM_003005), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD62P | CD62 | GMP-140 | GMRP | Granule membrane protein 140 | LECAM3 | P-selectin | SELP | PSEL | CD62P antigen | GMP140 | Granulocyte membrane protein | GRMP | PADGEM
- Origin Species
- Human
- NCBI GenBank
- NM_003005
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 90.78 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MANCQIAILY QRFQRVVFGI SQLLCFSALI SELTNQKEVA AWTYHYSTKA YSWNISRKYC61QNRYTDLVAI QNKNEIDYLN KVLPYYSSYY WIGIRKNNKT WTWVGTKKAL TNEAENWADN121EPNNKRNNED CVEIYIKSPS APGKWNDEHC LKKKHALCYT ASCQDMSCSK QGECLETIGN181YTCSCYPGFY GPECEYVREC GELELPQHVL MNCSHPLGNF SFNSQCSFHC TDGYQVNGPS241KLECLASGIW TNKPPQCLAA QCPPLKIPER GNMTCLHSAK AFQHQSSCSF SCEEGFALVG301PEVVQCTASG VWTAPAPVCK AVQCQHLEAP SEGTMDCVHP LTAFAYGSSC KFECQPGYRV361RGLDMLRCID SGHWSAPLPT CEAISCEPLE SPVHGSMDCS PSLRAFQYDT NCSFRCAEGF421MLRGADIVRC DNLGQWTAPA PVCQALQCQD LPVPNEARVN CSHPFGAFRY QSVCSFTCNE481GLLLVGASVL QCLATGNWNS VPPECQAIPC TPLLSPQNGT MTCVRPLGSS SYKSTCQFIC541DEGYSLSGPE RLDCTRSGRW TDSPPMCEAI KCPELFAPEQ GSLDCSDTRG EFNVGSTCHF601SCNNGFKLEG PNNVECTTSG RWSATPPTCK GIASLPTPGV QCPALTTPGQ GTMYCRHHPG661TFGFNTTCYF GCNAGFTLIG DSTLSCRPSG QWTAVTPACR AVKCSELHVN KPIAMNCSNL721WGNFSYGSIC SFHCLEGQLL NGSAQTACQE NGHWSTTVPT CQGPLTIQEA LTYFGGAVAS781TIGLIMGGTL LALLRKRFRQ KDDGKCPLNP HSHLGTYGVF TNAAFDPSPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
829 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.