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SEL1L3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SEL1L3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G101302-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEL1L3 (NM_015187), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0746 | Sel-1L3 | SEL1L3 | Protein sel-1 homolog 3
Origin Species
Human
NCBI GenBank
NM_015187
SwissProt
Q68CR1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111.71 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVYRDDYFIR HSISVSAVIV RAWITHKYSG RDWNVKWEEN LLHAVAKNYT LLQTIPPFER
61PFKDHQVCLE WNMGYIWNLR ANRIPQCPLE NDVVALLGFP YASSGENTGI VKKFPRFRNR
121ELEATRRQRM DYPVFTVSLW LYLLHYCKAN LCGILYFVDS NEMYGTPSVF LTEEGYLHIQ
181MHLVKGEDLA VKTKFIIPLK EWFRLDISFN GGQIVVTTSI GQDLKSYHNQ TISFREDFHY
241NDTAGYFIIG GSRYVAGIEG FFGPLKYYRL RSLHPAQIFN PLLEKQLAEQ IKLYYERCAE
301VQEIVSVYAS AAKHGGERQE ACHLHNSYLD LQRRYGRPSM CRAFPWEKEL KDKHPSLFQA
361LLEMDLLTVP RNQNESVSEI GGKIFEKAVK RLSSIDGLHQ ISSIVPFLTD SSCCGYHKAS
421YYLAVFYETG LNVPRDQLQG MLYSLVGGQG SERLSSMNLG YKHYQGIDNY PLDWELSYAY
481YSNIATKTPL DQHTLQGDQA YVETIRLKDD EILKVQTKED GDVFMWLKHE ATRGNAAAQQ
541RLAQMLFWGQ QGVAKNPEAA IEWYAKGALE TEDPALIYDY AIVLFKGQGV KKNRRLALEL
601MKKAASKGLH QAVNGLGWYY HKFKKNYAKA AKYWLKAEEM GNPDASYNLG VLHLDGIFPG
661VPGRNQTLAG EIFHKAAQGG HMEGTLWCSL YYITGNLETF PRDPEKAVVW AKHVAEKNGY
721LGHVIRKGLN AYLEGSWHEA LLYYVLAAET GIEVSQTNLA HICEERPDLA RRYLGVNCVW
781RYYNFSVFQI DAPSFAYLKM GDLYYYGHQN QSQDLELSVQ MYAQAALDGD SQGFFNLALL
841IEEGTIIPHH ILDFLEIDST LHSNNISILQ ELYERCWSHS NEESFSPCSL AWLYLHLRLL
901WGAILHSALI YFLGTFLLSI LIAWTVQYFQ SVSASDPPPR PSQASPDTAT STASPAVTPA
961ADASDQDQPT VTNNPEPRG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

979 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.