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SECISBP2L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SECISBP2L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SECISBP2L antibodies →
$504.00each
Catalog Number: LS-G113745-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SECISBP2L (NM_014701), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
D87445
SwissProt
Q93073

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
116.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDRAPTEQNV KLSAEVEPFI PQKKSPDTFM IPMALPNDNG SVSGVEPTPI PSYLITCYPF
61VQENQSNRQF PLYNNDIRWQ QPNPNPTGPY FAYPIISAQP PVSTEYTYYQ LMPAPCAQVM
121GFYHPFPTPY SNTFQAANTV NAITTECTER PSQLGQVFPL SSHRSRNSNR GSVVPKQQLL
181QQHIKSKRPL VKNVATQKET NAAGPDSRSK IVLLVDASQQ TDFPSDIANK SLSETTATML
241WKSKGRRRRA SHPTAESSSE QGASEADIDS DSGYCSPKHS NNQPAAGALR NPDSGTMNHV
301ESSMCAGGVN WSNVTCQATQ KKPWMEKNQT FSRGGRQTEQ RNNSQDEDGF QELNENGNAK
361DENIQQKLSS KVLDDLPENS PINIVQTPIP ITTSVPKRAK SQKKKALAAA LATAQEYSEI
421SMEQKKLQEA LSKAAGKKNK TPVQLDLGDM LAALEKQQQA MKARQITNTR PLSYTVVTAA
481SFHTKDSTNR KPLTKSQPCL TSFNSVDIAS SKAKKGKEKE IAKLKRPTAL KKVILKEREE
541KKGRLTVDHN LLGSEEPTEM HLDFIDDLPQ EIVSQEDTGL SMPSDTSLSP ASQNSPYCMT
601PVSQGSPASS GIGSPMASST ITKIHSKRFR EYCNQVLCKE IDECVTLLLQ ELVSFQERIY
661QKDPVRAKAR RRLVMGLREV TKHMKLNKIK CVIISPNCEK IQSKGGLDEA LYNVIAMARE
721QEIPFVFALG RKALGRCVNK LVPVSVVGIF NYFGAESLFN KLVELTEEAR KAYKDMVAAM
781EQEQAEEALK NVKKVPHHMG HSRNPSAASA ISFCSVISEP ISEVNEKEYE TNWRNMVETS
841DGLEASENEK EVSCKHSTSE KPSKLPFDTP PIGKQPSLVA TGSTTSATSA GKSTASDKEE
901VKPDDLEWAS QQSTETGSLD GSCRDLLNSS ITSTTSTLVP GMLEEEEDED EEEEEDYTHE
961PISVEVQLNS RIESWVSETQ RTMETLQLGK TLNGSEEDNV EQSGEEEAEA PEVLEPGMDS
1021EAWTADQQAS PGQQKSSNCS SLNKEHSDSN YTTQTT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1056 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.