Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SECISBP2 / SBP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110022-100
Catalog Number: LS-G110022
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SECISBP2 (NM_024077), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SECIS binding protein 2 | SECISBP2 | SBP2 | SECIS-binding protein 2
- Origin Species
- Human
- NCBI GenBank
- NM_024077
- SwissProt
- Q96T21
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 95.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASEGPREPE SEGIKLSADV KPFVPRFAGL NVAWLESSEA CVFPSSAATY YPFVQEPPVT61EQKIYTEDMA FGASTFPPQY LSSEITLHPY AYSPYTLDST QNVYSVPGSQ YLYNQPSCYR121GFQTVKHRNE NTCPLPQEMK ALFKKKTYDE KKTYDQQKFD SERADGTISS EIKSARGSHH181LSIYAENSLK SDGYHKRTDR KSRIIAKNVS TSKPEFEFTT LDFPELQGAE NNMSEIQKQP241KWGPVHSVST DISLLREVVK PAAVLSKGEI VVKNNPNESV TANAATNSPS CTRELSWTPM301GYVVRQTLST ELSAAPKNVT SMINLKTIAS SADPKNVSIP SSEALSSDPS YNKEKHIIHP361TQKSKASQGS DLEQNEASRK NKKKKEKSTS KYEVLTVQEP PRIEDAEEFP NLAVASERRD421RIETPKFQSK QQPQDNFKNN VKKSQLPVQL DLGGMLTALE KKQHSQHAKQ SSKPVVVSVG481AVPVLSKECA SGERGRRMSQ MKTPHNPLDS SAPLMKKGKQ REIPKAKKPT SLKKIILKER541QERKQRLQEN AVGPAFTSDD TQDGESGGDD QFPEQAELSG PEGMDELIST PSVEDKSEEP601PGTELQRDTE ASHLAPNHTT FPKIHSRRFR DYCSQMLSKE VDACVTDLLK ELVRFQDRMY661QKDPVKAKTK RRLVLGLREV LKHLKLKKLK CVIISPNCEK IQSKGGLDDT LHTIIDYACE721QNIPFVFALN RKALGRSLNK AVPVSVVGIF SYDGAQDQFH KMVELTVAAR QAYKTMLENV781QQELVGEPRP QAPPSLPTQG PSCPAEDGPP ALKEKEEPHY IEIWKKHLEA YSGCTLELEE841SLEASTSQMM NLNLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
854 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.