Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEC62 / TP-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117457-100
Catalog Number: LS-G117457
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEC62 (NM_003262), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Dtrp1 | HTP-1 | HTP1 | SEC62 | TP-1 | Translocation protein SEC62 | TLOC1 | SEC62 homolog (S. cerevisiae) | Translocation protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_003262
- SwissProt
- Q99442
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAERRRHKKR IQEVGEPSKE EKAVAKYLRF NCPTKSTNMM GHRVDYFIAS KAVDCLLDSK61WAKAKKGEEA LFTTRESVVD YCNRLLKKQF FHRALKVMKM KYDKDIKKEK DKGKAESGKE121EDKKSKKENI KDEKTKKEKE KKKDGEKEES KKEETPGTPK KKETKKKFKL EPHDDQVFLD181GNEVYVWIYD PVHFKTFVMG LILVIAVIAA TLFPLWPAEM RVGVYYLSVG AGCFVASILL241LAVARCILFL IIWLITGGRH HFWFLPNLTA DVGFIDSFRP LYTHEYKGPK ADLKKDEKSE301TKKQQKSDSE EKSDSEKKED EEGKVGPGNH GTEGSGGERH SDTDSDRRED DRSQHSSGNG361NDFEMITKEE LEQQTDGDCE EDEEEENDGE TPKSSHEKSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
399 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.