LSBio
SEC24D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SEC24D Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SEC24D antibodies →
$504.00each
Catalog Number: LS-G113695-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEC24D (SEC24D) (NM_014822), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0755 | SEC24-related protein D | SEC24D
Origin Species
Human
NCBI GenBank
NM_014822
SwissProt
O94855

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
112.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSQQGYVATP PYSQPQPGIG LSPPHYGHYG DPSHTASPTG MMKPAGPLGA TATRGMLPPG
61PPPPGPHQFG QNGAHATGHP PQRFPGPPPV NNVASSHAPY QPSAQSSYPG PISTSSVTQL
121GSQLSAMQIN SYGSGMAPPS QGPPGPLSAT SLQTPPRPPQ PSILQPGSQV LPPPPTTLNG
181PGASPLPLPM YRPDGLSGPP PPNAQYQPPP LPGQTLGAGY PPQQAANSGP QMAGAQLSYP
241GGFPGGPAQM AGPPQPQKKL DPDSIPSPIQ VIENDRASRG GQVYATNTRG QIPPLVTTDC
301MIQDQGNASP RFIRCTTYCF PCTSDMAKQA QIPLAAVIKP FATIPSNESP LYLVNHGESG
361PVRCNRCKAY MCPFMQFIEG GRRYQCGFCN CVNDVPPFYF QHLDHIGRRL DHYEKPELSL
421GSYEYVATLD YCRKSKPPNP PAFIFMIDVS YSNIKNGLVK LICEELKTML EKIPKEEQEE
481TSAIRVGFIT YNKVLHFFNV KSNLAQPQMM VVTDVGEVFV PLLDGFLVNY QESQSVIHNL
541LDQIPDMFAD SNENETVFAP VIQAGMEALK AADCPGKLFI FHSSLPTAEA PGKLKNRDDK
601KLVNTDKEKI LFQPQTNVYD SLAKDCVAHG CSVTLFLFPS QYVDVASLGL VPQLTGGTLY
661KYNNFQMHLD RQQFLNDLRN DIEKKIGFDA IMRVRTSTGF RATDFFGGIL MNNTTDVEMA
721AIDCDKAVTV EFKHDDKLSE DSGALIQCAV LYTTISGQRR LRIHNLGLNC SSQLADLYKS
781CETDALINFF AKSAFKAVLH QPLKVIREIL VNQTAHMLAC YRKNCASPSA ASQLILPDSM
841KVLPVYMNCL LKNCVLLSRP EISTDERAYQ RQLVMTMGVA DSQLFFYPQL LPIHTLDVKS
901TMLPAAVRCS ESRLSEEGIF LLANGLHMFL WLGVSSPPEL IQGIFNVPSF AHINTDMTLL
961PEVGNPYSQQ LRMIMGIIQQ KRPYSMKLTI VKQREQPEMV FRQFLVEDKG LYGGSSYVDF
1021LCCVHKEICQ LLN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1033 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.