LSBio
SEC24B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SEC24B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SEC24B antibodies →
$725.00each
Catalog Number: LS-G119412-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEC24B (NM_001042734), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SEC24 | Secretory protein 24b | SEC24-related protein B | SEC24B | Secretory protein 24
Origin Species
Human
NCBI GenBank
NM_006323
SwissProt
O95487

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSAPAGSSHP AASARIPPKF GGAAVSGAAA PAGPGAGPAP HQQNGPAQNQ MQVPSGYGLH
61HQNYIAPSGH YSQGPGKMTS LPLDTQCGDY YSALYTVPTQ NVTPNTVNQQ PGAQQLYSRG
121PPAPHIVGST LGSFQGAASS ASHLHTSASQ PYSSFVNHYN SPAMYSASSS VASQGFPSTC
181GHYAMSTVSN AAYPSVSYPS LPAGDTYGQM FTSQNAPTVR PVKDNSFSGQ NTAISHPSPL
241PPLPSQQHHQ QQSLSGYSTL TWSSPGLPST QDNLIRNHTG SLAVANNNPT ITVADSLSCP
301VMQNVQPPKS SPVVSTVLSG SSGSSSTRTP PTANHPVEPV TSVTQPSELL QQKGVDSSST
361TSSASPMPNS YDALEGGSYP DMLSSSASSP APDPAPEPDP ASAPAPASAP APVVPQPSKM
421AKPFGYGYPT LQPGYQNATA PLISGVQPSN PVYSGFQQYP QQYPGVNQLS SSIGGLSLQS
481SPQPESLRPV NLTQERNILP MTPVWAPVPN LNADLKKLNC SPDSFRCTLT NIPQTQALLN
541KAKLPLGLLL HPFRDLTQLP VITSNTIVRC RSCRTYINPF VSFIDQRRWK CNLCYRVNDV
601PEEFMYNPLT RSYGEPHKRP EVQNSTVEFI ASSDYMLRPP QPAVYLFVLD VSHNAVEAGY
661LTILCQSLLE NLDKLPGDSR TRIGFMTFDS TIHFYNLQEG LSQPQMLIVS DIDDVFLPTP
721DSLLVNLYES KELIKDLLNA LPNMFTNTRE THSALGPALQ AAFKLMSPTG GRVSVFQTQL
781PSLGAGLLQS REDPNQRSST KVVQHLGPAT DFYKKLALDC SGQQTAVDLF LLSSQYSDLA
841SLACMSKYSA GCIYYYPSFH YTHNPSQAEK LQKDLKRYLT RKIGFEAVMR IRCTKGLSMH
901TFHGNFFVRS TDLLSLANIN PDAGFAVQLS IEESLTDTSL VCFQTALLYT SSKGERRIRV
961HTLCLPVVSS LADVYAGVDV QAAICLLANM AVDRSVSSSL SDARDALVNA VVDSLSAYGS
1021TVSNLQHSAL MAPSSLKLFP LYVLALLKQK AFRTGTSTRL DDRVYAMCQI KSQPLVHLMK
1081MIHPNLYRID RLTDEGAVHV NDRIVPQPPL QKLSAEKLTR EGAFLMDCGS VFYIWVGKGC
1141DNNFIEDVLG YTNFASIPQK MTHLPELDTL SSERARSFIT WLRDSRPLSP ILHIVKDESP
1201AKAEFFQHLI EDRTEAAFSY YEFLLHVQQQ ICK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1233 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.