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SEC24A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SEC24A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SEC24A antibodies →
$725.00each
Catalog Number: LS-G110512-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEC24A (NM_021982), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SEC24-related protein A | SEC24A | Secretory protein 24 a isoform
Origin Species
Human
NCBI GenBank
AJ131244
SwissProt
O95486

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
119.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSQPGIPASG GAPASLQAQN GAALASGSPY TNGPVQNALL SSQESVSQGY NFQLPGSYPH
61PIPAKTLNPV SGQSNYGGSQ GSGQTLNRPP VASNPVTPSL HSGPAPRMPL PASQNPATTP
121MPSSSFLPEA NLPPPLNWQY NYPSTASQTN HCPRASSQPT VSGNTSLTTN HQYVSSGYPS
181LQNSFIKSGP SVPPLVNPPL PTTFQPGAPH GPPPAGGPPP VRALTPLTSS YRDVPQPLFN
241SAVNQEGITS NTNNGSMVVH SSYDEIEGGG LLATPQLTNK NPKMSRSVGY SYPSLPPGYQ
301NTTPPGATGV PPSSLNYPSG PQAFTQTPLG ANHLTTSMSG LSLQPEGLRV VNLLQERNML
361PSTPLKPPVP NLHEDIQKLN CNPELFRCTL TSIPQTQALL NKAKLPLGLL LHPFKDLVQL
421PVVTSSTIVR CRSCRTYINP FVSFLDQRRW KCNLCYRVND VPEEFLYNPL TRVYGEPHRR
481PEVQNATIEF MAPSEYMLRP PQPPVYLFVF DVSHNAVETG YLNSVCQSLL DNLDLLPGNT
541RTKIGFITFD STIHFYGLQE SLSQPQMLIV SDIEDVFIPM PENLLVNLNE SKELVQDLLK
601TLPQMFTKTL ETQSALGPAL QAAFKLMSPT GGRMSVFQTQ LPTLGVGALK PREEPNHRSS
661AKDIHMTPST DFYKKLALDC SGQQVAVDLF LLSGQYSDLA SLGCISRYSA GSVYYYPSYH
721HQHNPVQVQK LQKELQRYLT RKIGFEAVMR IRCTKGLSIH TFHGNFFVRS TDLLSLPNVN
781PDAGYAVQMS VEESLTDTQL VSFQSALLYT SSKGERRIRV HTLCLPVVST LNDVFLGADV
841QAISGLLANM AVDRSMTASL SDARDALVNA VIDSLSAYRS SVLSNQQPGL MVPFSLRLFP
901LFVLALLKQK SFQTGTNARL DERIFAMCQV KNQPLVYLML TTHPSLYRVD NLSDEGALNI
961SDRTIPQPPI LQLSVEKLSR DGAFLMDAGS VLMLWVGKNC TQNFLSQVLG VQNYASIPQP
1021MTDLPELDTP ESARIIAFIS WLREQRPFFP ILYVIRDESP MKANFLQNMI EDRTESALSY
1081YEFLLHIQQQ VNK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1093 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.